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Biocompatibility of synthesised nano-porous anodic Aluminium oxide membranes for use as a cell culture substrate for Madin-Darby Canine Kidneys cells: A preliminary study

机译:合成的纳米多孔阳极氧化铝膜用作Madin-Darby犬肾细胞的细胞培养基质的生物相容性:初步研究

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摘要

In this study we investigate for the first time the biomedical potential of using a membrane made from anodic aluminium oxide (AAO) for culturing the Madin-Darby Canine Kidney (MDCK) epithelial cell line. Nano-porous aluminium oxide membranes exhibit interesting properties such as high porosity, which allows the exchange of molecules and nutrients across the membrane and can be made with highly specific pore sizes that can be preselected by adjusting the controlling parameters of a temperature controlled two-step anodization process. The cellular response and interactions of the MDCK cell line with the synthesised nano-porous AAO membrane, a commercially available membrane and a glass control were assessed by investigating cell adhesion, morphology and proliferation.
机译:在这项研究中,我们首次研究了使用由阳极氧化铝(AAO)制成的膜培养Madin-Darby犬肾脏(MDCK)上皮细胞系的生物医学潜力。纳米多孔氧化铝膜表现出令人感兴趣的特性,例如高孔隙率,可以在膜上交换分子和营养素,并且可以制成特定的孔径,可以通过调节温度控制两步法的控制参数来预先选择阳极氧化过程。通过研究细胞粘附,形态和增殖来评估MDCK细胞系与合成的纳米多孔AAO膜,可商购的膜和玻璃对照的细胞应答和相互作用。

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